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Premier Biosoft primer premier software
Primer Premier Software, supplied by Premier Biosoft, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primer+premier+software/0+5+premier+primer/pmc13129384-112-9-14
Average 86 stars, based on 1 article reviews
primer premier software - by Bioz Stars, 2026-11
86/100 stars

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Related Articles

Sequencing:

Article Title: TaLACS3 positively regulates wheat resistance to Puccinia striiformis f. sp. tritici by modulating ROS accumulation.
Article Snippet: The full-length sequence of TaLACS3-7D (XM_044582519.1) was retrieved from the National Center for Biotechnology Information (NCBI) database (https://www.ncbi.nlm.nih.gov/). .. Gene-specific primers (TaLACS3-clone-F/R, Table 1) targeting the coding sequence (CDs) of TaLACS3-7D were designed using Primer Premier software (version 6.0, Premier Biosoft, Palo Alto, CA, USA). ..

Article Title: The AP-1 Sigma Subunit Gene PsAP1 Acts as a Key Pathogenicity Factor by Regulating Metabolic Reprogramming in Puccinia striiformis f. sp. tritici .
Article Snippet: Total RNA was extracted using the Polysaccharide and Polyphenol Plant Total RNA Extraction Kit (Tiangen, Beijing, China), and cDNA was synthesized using a reverse transcription kit (Vazyme, Nanjing, China). .. The full-length sequence of PsAP1 (XM_047955384.1) was downloaded from the NCBI website (https://www.ncbi. nlm.nih.gov/), and gene-specific primers for amplifying the coding sequence (CDS) region of PsAP1 were designed using Primer Premier software (version 6.0, Premier Biosoft, Palo Alto, CA, USA). ..

Article Title: The AP-1 Sigma Subunit Gene PsAP1 Acts as a Key Pathogenicity Factor by Regulating Metabolic Reprogramming in Puccinia striiformis f. sp. tritici
Article Snippet: Total RNA was extracted using the Polysaccharide and Polyphenol Plant Total RNA Extraction Kit (Tiangen, Beijing, China), and cDNA was synthesized using a reverse transcription kit (Vazyme, Nanjing, China). .. The full-length sequence of PsAP1 ( XM_047955384.1 ) was downloaded from the NCBI website ( https://www.ncbi.nlm.nih.gov/ ), and gene-specific primers for amplifying the coding sequence (CDS) region of PsAP1 were designed using Primer Premier software (version 6.0, Premier Biosoft, Palo Alto, CA, USA). ..

Software:

Article Title: TaLACS3 positively regulates wheat resistance to Puccinia striiformis f. sp. tritici by modulating ROS accumulation.
Article Snippet: The full-length sequence of TaLACS3-7D (XM_044582519.1) was retrieved from the National Center for Biotechnology Information (NCBI) database (https://www.ncbi.nlm.nih.gov/). .. Gene-specific primers (TaLACS3-clone-F/R, Table 1) targeting the coding sequence (CDs) of TaLACS3-7D were designed using Primer Premier software (version 6.0, Premier Biosoft, Palo Alto, CA, USA). ..

Article Title: Targeting TLR4 Attenuates Endometriosis Progression by Suppressing NF-κB/NLRP3 Inflammasome Activation and Angiogenesis
Article Snippet: .. Primers were designed with Primer Premier software (Premier Biosoft, Palo Alto, CA, USA), and sequences are listed in . qRT-PCR was performed with SYBR Green I Master Mix (Takara, Dalian, China), using GAPDH as the reference gene for normalization. ..

Article Title: Targeting TLR4 Attenuates Endometriosis Progression by Suppressing NF-κB/NLRP3 Inflammasome Activation and Angiogenesis.
Article Snippet: Only RNA with OD260/OD280 ratio 1.8–2.0 was used to ensure purity. .. Primers were designed with Primer Premier software (Premier Biosoft, Palo Alto, CA, USA), and sequences are listed in Table 4. qRT-PCR was performed with SYBR Green I Master Mix (Takara, Dalian, China), using GAPDH as the reference gene for normalization. ..

Article Title: Transcriptomics identified crucial candidate genes and pathways regulating early testicular development of drakes in the hypothalamic-pituitary-gonadal/thyroid axes
Article Snippet: The network was visualized with Cytoscape 3.9.1, with nodes representing proteins and edges indicating predicted physical interactions. .. Total RNA extracted from the hypothalamus, pituitary, thyroid, and testis was reverse transcribed into cDNA using the HiScript III RT SuperMix for qPCR (+ gDNA wiper) (Vazyme, Jiangsu, China), and the Primer Premier software (PREMIER Biosoft, San Francisco, CA, USA) was used to design primers (Table ), and the NCBI online website ( https://www.ncbi.nlm.nih.gov/tools/primer-blast/index.cgi ) was used to determine the specificity of the primers. qRT-PCR was performed using the BioRad CFX96 real-time PCR detection system (BioRad, Hercules, CA, USA), and 3 replicates were done for each sample. β -actin and GAPDH were selected as reference genes. .. Total RNA extracted from the hypothalamus, pituitary, thyroid, and testis was reverse transcribed into cDNA using the HiScript III RT SuperMix for qPCR (+ gDNA wiper) (Vazyme, Jiangsu, China), and the Primer Premier software (PREMIER Biosoft, San Francisco, CA, USA) was used to design primers (Table ), and the NCBI online website ( https://www.ncbi.nlm.nih.gov/tools/primer-blast/index.cgi ) was used to determine the specificity of the primers.

Article Title: The AP-1 Sigma Subunit Gene PsAP1 Acts as a Key Pathogenicity Factor by Regulating Metabolic Reprogramming in Puccinia striiformis f. sp. tritici .
Article Snippet: Total RNA was extracted using the Polysaccharide and Polyphenol Plant Total RNA Extraction Kit (Tiangen, Beijing, China), and cDNA was synthesized using a reverse transcription kit (Vazyme, Nanjing, China). .. The full-length sequence of PsAP1 (XM_047955384.1) was downloaded from the NCBI website (https://www.ncbi. nlm.nih.gov/), and gene-specific primers for amplifying the coding sequence (CDS) region of PsAP1 were designed using Primer Premier software (version 6.0, Premier Biosoft, Palo Alto, CA, USA). ..

Article Title: TIAM2 and ADCY7 polymorphisms and their association with feed efficiency in Huaibei partridge chickens
Article Snippet: Candidate single‐nucleotide polymorphisms ( SNPs ) were initially screened in the chicken genome on the Ensembl annotation database (GRCg6a), and the corresponding reference sequences were retrieved from the NCBI (National Center for Biotechnology Information). .. Gene-specific primers flanking the target regions were designed using Primer Premier software (version 5.0, PREMIER Biosoft International, Palo Alto, CA, USA). ..

Article Title: The AP-1 Sigma Subunit Gene PsAP1 Acts as a Key Pathogenicity Factor by Regulating Metabolic Reprogramming in Puccinia striiformis f. sp. tritici
Article Snippet: Total RNA was extracted using the Polysaccharide and Polyphenol Plant Total RNA Extraction Kit (Tiangen, Beijing, China), and cDNA was synthesized using a reverse transcription kit (Vazyme, Nanjing, China). .. The full-length sequence of PsAP1 ( XM_047955384.1 ) was downloaded from the NCBI website ( https://www.ncbi.nlm.nih.gov/ ), and gene-specific primers for amplifying the coding sequence (CDS) region of PsAP1 were designed using Primer Premier software (version 6.0, Premier Biosoft, Palo Alto, CA, USA). ..

Quantitative RT-PCR:

Article Title: Targeting TLR4 Attenuates Endometriosis Progression by Suppressing NF-κB/NLRP3 Inflammasome Activation and Angiogenesis
Article Snippet: .. Primers were designed with Primer Premier software (Premier Biosoft, Palo Alto, CA, USA), and sequences are listed in . qRT-PCR was performed with SYBR Green I Master Mix (Takara, Dalian, China), using GAPDH as the reference gene for normalization. ..

Article Title: Targeting TLR4 Attenuates Endometriosis Progression by Suppressing NF-κB/NLRP3 Inflammasome Activation and Angiogenesis.
Article Snippet: Only RNA with OD260/OD280 ratio 1.8–2.0 was used to ensure purity. .. Primers were designed with Primer Premier software (Premier Biosoft, Palo Alto, CA, USA), and sequences are listed in Table 4. qRT-PCR was performed with SYBR Green I Master Mix (Takara, Dalian, China), using GAPDH as the reference gene for normalization. ..

Article Title: Transcriptomics identified crucial candidate genes and pathways regulating early testicular development of drakes in the hypothalamic-pituitary-gonadal/thyroid axes
Article Snippet: The network was visualized with Cytoscape 3.9.1, with nodes representing proteins and edges indicating predicted physical interactions. .. Total RNA extracted from the hypothalamus, pituitary, thyroid, and testis was reverse transcribed into cDNA using the HiScript III RT SuperMix for qPCR (+ gDNA wiper) (Vazyme, Jiangsu, China), and the Primer Premier software (PREMIER Biosoft, San Francisco, CA, USA) was used to design primers (Table ), and the NCBI online website ( https://www.ncbi.nlm.nih.gov/tools/primer-blast/index.cgi ) was used to determine the specificity of the primers. qRT-PCR was performed using the BioRad CFX96 real-time PCR detection system (BioRad, Hercules, CA, USA), and 3 replicates were done for each sample. β -actin and GAPDH were selected as reference genes. .. Total RNA extracted from the hypothalamus, pituitary, thyroid, and testis was reverse transcribed into cDNA using the HiScript III RT SuperMix for qPCR (+ gDNA wiper) (Vazyme, Jiangsu, China), and the Primer Premier software (PREMIER Biosoft, San Francisco, CA, USA) was used to design primers (Table ), and the NCBI online website ( https://www.ncbi.nlm.nih.gov/tools/primer-blast/index.cgi ) was used to determine the specificity of the primers.

SYBR Green Assay:

Article Title: Targeting TLR4 Attenuates Endometriosis Progression by Suppressing NF-κB/NLRP3 Inflammasome Activation and Angiogenesis
Article Snippet: .. Primers were designed with Primer Premier software (Premier Biosoft, Palo Alto, CA, USA), and sequences are listed in . qRT-PCR was performed with SYBR Green I Master Mix (Takara, Dalian, China), using GAPDH as the reference gene for normalization. ..

Article Title: Targeting TLR4 Attenuates Endometriosis Progression by Suppressing NF-κB/NLRP3 Inflammasome Activation and Angiogenesis.
Article Snippet: Only RNA with OD260/OD280 ratio 1.8–2.0 was used to ensure purity. .. Primers were designed with Primer Premier software (Premier Biosoft, Palo Alto, CA, USA), and sequences are listed in Table 4. qRT-PCR was performed with SYBR Green I Master Mix (Takara, Dalian, China), using GAPDH as the reference gene for normalization. ..

Reverse Transcription:

Article Title: Transcriptomics identified crucial candidate genes and pathways regulating early testicular development of drakes in the hypothalamic-pituitary-gonadal/thyroid axes
Article Snippet: The network was visualized with Cytoscape 3.9.1, with nodes representing proteins and edges indicating predicted physical interactions. .. Total RNA extracted from the hypothalamus, pituitary, thyroid, and testis was reverse transcribed into cDNA using the HiScript III RT SuperMix for qPCR (+ gDNA wiper) (Vazyme, Jiangsu, China), and the Primer Premier software (PREMIER Biosoft, San Francisco, CA, USA) was used to design primers (Table ), and the NCBI online website ( https://www.ncbi.nlm.nih.gov/tools/primer-blast/index.cgi ) was used to determine the specificity of the primers. qRT-PCR was performed using the BioRad CFX96 real-time PCR detection system (BioRad, Hercules, CA, USA), and 3 replicates were done for each sample. β -actin and GAPDH were selected as reference genes. .. Total RNA extracted from the hypothalamus, pituitary, thyroid, and testis was reverse transcribed into cDNA using the HiScript III RT SuperMix for qPCR (+ gDNA wiper) (Vazyme, Jiangsu, China), and the Primer Premier software (PREMIER Biosoft, San Francisco, CA, USA) was used to design primers (Table ), and the NCBI online website ( https://www.ncbi.nlm.nih.gov/tools/primer-blast/index.cgi ) was used to determine the specificity of the primers.

Real-time Polymerase Chain Reaction:

Article Title: Transcriptomics identified crucial candidate genes and pathways regulating early testicular development of drakes in the hypothalamic-pituitary-gonadal/thyroid axes
Article Snippet: The network was visualized with Cytoscape 3.9.1, with nodes representing proteins and edges indicating predicted physical interactions. .. Total RNA extracted from the hypothalamus, pituitary, thyroid, and testis was reverse transcribed into cDNA using the HiScript III RT SuperMix for qPCR (+ gDNA wiper) (Vazyme, Jiangsu, China), and the Primer Premier software (PREMIER Biosoft, San Francisco, CA, USA) was used to design primers (Table ), and the NCBI online website ( https://www.ncbi.nlm.nih.gov/tools/primer-blast/index.cgi ) was used to determine the specificity of the primers. qRT-PCR was performed using the BioRad CFX96 real-time PCR detection system (BioRad, Hercules, CA, USA), and 3 replicates were done for each sample. β -actin and GAPDH were selected as reference genes. .. Total RNA extracted from the hypothalamus, pituitary, thyroid, and testis was reverse transcribed into cDNA using the HiScript III RT SuperMix for qPCR (+ gDNA wiper) (Vazyme, Jiangsu, China), and the Primer Premier software (PREMIER Biosoft, San Francisco, CA, USA) was used to design primers (Table ), and the NCBI online website ( https://www.ncbi.nlm.nih.gov/tools/primer-blast/index.cgi ) was used to determine the specificity of the primers.

other:

Article Title: Penicillin–streptomycin influences macrophage mechanical properties and microenvironment mechano-sensation
Article Snippet: qRT-PCR assay was performed using the SYBR premix Ex Taq reagent (Takara) with a CFX Connect Real-Time PCR Detection System (Bio-Rad, Hercules, CA, USA).



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